Central Tissue Culture Lab
The Central Tissue Culture Laboratory serves as a specialized facility for cell culture-based research, vaccine production support, and diagnostic applications. It provides a sterile and controlled environment for maintaining cell lines, cultivating viruses, and performing cellular assays essential to vaccine development and quality evaluation.
Overview
The Central Tissue Culture Laboratory plays a vital role in supporting biological production, vaccine development, and cell-based research. It maintains continuous cell lines and primary cultures used for virus propagation, vaccine potency testing, and serological assays.
The laboratory’s infrastructure enables precise control of environmental conditions and ensures biosafety compliance in handling live cultures and infectious materials.
Core Functions
- • Maintenance and propagation of animal cell lines
- • Preparation of cell cultures for viral vaccine production
- • Cytotoxicity and viability testing of biologicals
- • Support to diagnostic divisions for virus isolation and assay development
- • Standardization of in-vitro testing protocols
- • Preservation and revival of cell banks under controlled conditions
Infrastructure and Facilities
The laboratory is equipped with advanced biosafety and environmental control systems for high-quality cell culture operations, including:
- • Class II Biosafety cabinets and laminar airflow benches
- • CO₂ incubators and refrigerated centrifuges
- • Inverted and phase-contrast microscopes for cell monitoring
- • Cryogenic storage and cell banking facilities
- • Automated media preparation and filtration units
- • Temperature and humidity monitoring with data logging systems
Research and Collaboration
The Central Tissue Culture Lab collaborates with production, quality control, and diagnostic units to develop new cell-based assays and improve vaccine yield and purity. It also supports national and international research programs involving cell line authentication, virus characterization, and cytological studies.
Quality and Compliance
All procedures in the laboratory follow Good Laboratory Practices (GLP) and institutional biosafety standards. Routine calibration, environmental monitoring, and documentation ensure traceability and reproducibility of cell-based experiments.
Mission and Commitment
The Central Tissue Culture Lab is committed to advancing scientific excellence in cell biology, vaccine development, and diagnostic innovation — ensuring that all biological research and production processes are supported by precision, safety, and integrity.