Hands-on Training: Cell Culture Techniques
The Hands-on Training Programme on Cell Culture Techniques provides students with practical experience in the cultivation, maintenance, and application of animal cell lines. This training builds essential laboratory skills for careers in vaccine research, diagnostics, and biotechnology-based industries.
Overview
This training introduces participants to the fundamental techniques involved in animal cell culture — a cornerstone of modern biomedical and vaccine research. Students gain exposure to aseptic techniques, cell maintenance, subculturing, and viability assessment.
The module emphasizes good laboratory practices, biosafety, and hands-on operation of key instruments used in cell culture laboratories.
Key Training Components
- • Introduction to cell culture principles and laboratory safety
- • Preparation of culture media and sterilization techniques
- • Maintenance of continuous cell lines and primary cultures
- • Subculturing, trypsinization, and cell counting procedures
- • Assessment of cell viability and contamination control
- • Cryopreservation and revival of cell stocks
Laboratory Infrastructure
Training is conducted in biosafety-compliant facilities featuring:
- • Class II biosafety cabinets and laminar airflow benches
- • CO₂ incubators and refrigerated centrifuges
- • Inverted and phase-contrast microscopes
- • Cryogenic storage and controlled freezing systems
- • Automated media filtration and sterilization units
Learning Outcomes
- • Develop proficiency in aseptic handling and culture maintenance
- • Understand the growth characteristics of animal cells
- • Perform subculturing and evaluate cell health and morphology
- • Apply biosafety protocols in live cell handling
- • Document culture processes and maintain traceable records
Duration and Eligibility
The training is offered for a duration of 1 to 2 weeks and is open to undergraduate and postgraduate students of Life Sciences, Biotechnology, Veterinary Sciences, and related disciplines. Limited seats are available to ensure hands-on supervision and practical engagement.
Objective and Mission
The program’s mission is to develop skilled professionals in cell culture technology by providing intensive practical training and exposure to industry-relevant laboratory protocols. Students are encouraged to apply theoretical knowledge to real-world research and diagnostic challenges.