Hands-on Training: Egg Inoculation & Immunological Techniques
The Hands-on Training Programme on Egg Inoculation and Immunological Techniques is designed to give students practical exposure to fundamental methods used in virology, vaccine development, and immunodiagnostics. Participants gain real-world experience in aseptic handling, virus cultivation, and immunoassay procedures under expert supervision.
Overview
This training program focuses on imparting hands-on experience in embryonated egg inoculation and basic immunological techniques commonly used in vaccine development and diagnostic assays. Participants learn to handle eggs aseptically, inoculate viral suspensions, and perform serological and immunoassay-based evaluations.
It aims to bridge theoretical knowledge and practical application, preparing students for research and technical roles in virology and immunology laboratories.
Key Training Components
- • Introduction to embryonated egg systems used in virology
- • Egg handling, candling, and sterilization techniques
- • Inoculation routes – chorioallantoic membrane (CAM), allantoic cavity, amniotic cavity, and yolk sac
- • Harvesting of viral fluids and processing for analysis
- • Principles and practice of immunological assays (ELISA, HA, HI tests)
- • Safety practices and biosafety level handling in virology labs
Laboratory Infrastructure
Training is conducted in well-equipped laboratories with facilities such as:
- • Dedicated egg incubation and inoculation rooms
- • Class II biosafety cabinets and laminar airflow units
- • CO₂ incubators and temperature-controlled egg storage systems
- • Microscopes and serological testing equipment
- • ELISA readers and automated immunoassay instruments
Learning Outcomes
- • Understand the role of embryonated eggs in vaccine research
- • Gain proficiency in safe inoculation and virus harvesting methods
- • Learn to perform and interpret immunological assays
- • Apply biosafety and documentation standards in laboratory practice
Duration and Eligibility
The training is typically offered for a duration of 1 to 2 weeks, open to undergraduate and postgraduate students of Life Sciences, Veterinary Sciences, Biotechnology, and Microbiology. Enrollment is limited to ensure personalized mentoring and practical exposure.
Objective and Mission
The mission of this training is to nurture technical competence and scientific curiosity among students by providing hands-on exposure to classical and modern techniques used in vaccine research and immunological testing.